Leaf-rust resistance gene sourced from wild relative of wheat. NBThighlight
). Isolates were propagated on seedlings of the susceptible wheat cultivar Thatcher. Freshly collected urediniospores were used for inoculation experiments. Inoculations were performed using a high-pressure air sprayer or a settling tower. For the spray inoculation, urediniospores were suspended in FC-43 oil and sprayed onto plants using a glass sprayer connected to a high-pressure air pipe. For the settling tower inoculation, plants were grown for 12 d.
The treated grains were planted into 18-well trays filled with Stender soil, 12 to 14 grains per well. Plants were grown in a greenhouse under speed-breeding conditions . Spikes from the first and second batches of TA5605 M1 plants were bulk collected. Single spikes were collected from the third batch of TA5605 M1 plants and from TcM1 plants.
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