Early prenatal alcoholexposure affects genes involved in embryonic development helsinkiuni BMCMedicine
] was used to perform gene-set enrichment analysis for significant differentially expressed genes. The GO knowledgebase was used as the source for identifying significantly enriched BP terms . Benjamini-Hochberg procedure was used to control for FDR.Normalized genome-wide DNAm data was compared to similarly adjusted mRNA-seq data to discover genes, which DNAm changes correlate with mRNA expression in the placenta and hESCs.
] with some modifications using H1 and Regea08/017 cell lines. Two replicates of both cell lines were used, which is four replicates of alcohol-exposed and control ChIP samples in total. Briefly, cells were cross-linked using 1% formaldehyde and sonicated with Bioruptor® Pico sonication device using optimized parameters 4 cycles of 30s on/90s off to generate DNA fragments of 300–600 bp. For immunoprecipitation, 0.
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