Team artificially re-creates cell 'skeletons' using strands of DNA uclnews NatureComms
DNA nanotube cytoskeleton DMPC/cholesterol protocells were added to 1 Chol strand containing a FAM fluorophore and left to incubate at 37 °C for 15 min. Next, either hairy DNA nanotubes or hairy fibers were added and the solution left for 15 min. The exoskeleton GUVs were then deposited into PBS and imaged using
time-series were collected across the whole, in the center, or at the edge of the synthetic tissue over the stated time frames. To generate tissue networks with exoskeleton protocells, the above procedure was followed, except FAM-labeled cholesterol fibers were added to the GUV solution and then deposited on the surface. To cross-link prototissues, Cy5-labeled hairy DNA fibers were carefully deposited onto the tissue network.
was used to quantify levels of proinflammatory cytokines interleukin-6 and tumor necrosis factor-alpha released in whole blood assays. DuoSet
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