Deep phenotyping and lifetime trajectories reveal limited effects of longevity regulators on the aging process in C57BL/6J mice - Nature Communications

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Deep phenotyping and lifetime trajectories reveal limited effects of longevity regulators on the aging process in C57BL/6J mice - Nature Communications
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Three approaches with a reputation for slowing aging processes proven largely ineffective NatureComms

. Cardiac electrical activity was measured through the paws of the animal staying on a shielded acquisition platform. Intervals and amplitude from at least 15 consecutive ECGs were averaged. Heart rate was calculated from peak detections. P, Q, R, S and T waves were analyzed such that unfiltered noise or motion artifacts were excluded. The corrected QT interval was calculated by dividing the QT interval by the square root of the preceding RR interval.

. We measured thickness and morphology of the retina, as well as morphology of the optical disk, fundus pigmentation and the number of main blood vessels.Eye size measurements were performed using the AC Master system . Briefly, anaesthetized mice were placed on a platform. Proper positioning of the animals was supported by light signals from six infrared LEDs arranged in a circle that must be placed in the center of the pupil. Eyes were treated with 1% atropine to ensure pupil dilation.

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