Single-cell transcriptomics analyses of pretreatment and post-treatment peripheral blood mononuclear cells from patients with refractory large B cell lymphoma treated with CD19 CART products reveal a role for CAR-Treg cells in treatment relapse.
Pseudobulk expression of CD8+CAR-T axi-cel IP cells for genes proposed to be response-associated by Deng et al. Fisher Exact test for association between response and denoted two clusters driven by putative memory- and dysfunction-associated genes is p = 1.
Extended Data Fig. 4 Detection of CD45 isoforms and T cell subset classification in 5’ RNA-sequencing data., Illustration of signal used by CD45 isoform detection model. For each read in an illustrative sample, a histogram of the fragment length assuming no splicing is shown. The distribution of reads beyond exon 3 become shifted if they come from an isoform lacking an upstream exon., A histogram of the expected fragment length after inference of each read is shown for the same sample in blue.
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