Biologists, chemical engineers collaborate to reveal complex cellular process inside petunias LifeAtPurdue NatureComms
) to produce a fusion protein with C-terminal green fluorescent protein , AtPIP1:GFP. The resulting constructs and an empty RFP vector were transformed intoleaves and imaged 48 h after. For plasmolysis, leaf discs were incubated in 20% sucrose for 60 min prior to imaging. Images were acquired using a Zeiss LSM-880 Upright Confocal system and operated using Zeiss Efficient Navigation -Black Edition software .
Colonies were verified for the presence of insert by PCR with the forward α factor primer 5´-TACTATTGCCAGCATTGCTGC-3´ and the reverse AOX1 primer 5´-GCAAATGGCATTCTGACATCC-3´, the latter is located downstream of 6×His-tag. A single positive colony for each PhnsLTP was inoculated in 10 mL Buffered Glycerol-complex Medium , 4 μg Lreached 2–6.
Deutschland Neuesten Nachrichten, Deutschland Schlagzeilen
Similar News:Sie können auch ähnliche Nachrichten wie diese lesen, die wir aus anderen Nachrichtenquellen gesammelt haben.
Attosecond-Angstrom free-electron-laser towards the cold beam limit - Nature CommunicationsFree-electron lasers (FELs) can produce bright X-ray pulses, but require high quality electron beams. Here the authors show how to generate and preserve ultrabright electron beams from plasma-based accelerators for ultra-compact, high-brightness X-ray FELs.
Weiterlesen »
Diverse behaviors in non-uniform chiral and non-chiral swarmalators - Nature CommunicationsPopulations of swarming coupled oscillators with inhomogeneous natural frequencies and chirality are relevant for active matter systems and micro-robotics. The authors model and analyze a variety of their self-organized behaviors that mimic natural and artificial micro-scale collective systems.
Weiterlesen »
Crocodile defensin (CpoBD13) antifungal activity via pH-dependent phospholipid targeting and membrane disruption - Nature CommunicationsDefensins are a class of host defence peptides that contribute to the immune system of eukaryotes. Here, the authors report the structure of saltwater crocodile defensin CpoBD13 and the mechanism of pH-dependent antifungal activity.
Weiterlesen »
Cyberattack on Dish Network leads to stolen data, internal communications issuesA cyberattack on Dish Network has impacted the company’s servers, customer call centers, online service desk, and other internal communications.
Weiterlesen »
Base editors - Nature BiotechnologyRecent patents relating to compositions and methods for targeted editing of nucleic acids.
Weiterlesen »
First bee vaccine - Nature BiotechnologyIn Brief: USDA gives conditional approval to a vaccine that protects honeybees from American foulbrood disease. The first vaccine to protect insects, it could mark a step-change in pest control methods
Weiterlesen »