Metatranscriptome mining of over five thousand datasets from various environments expands what we know about the diversity of the global virome. NBThighlight
Because the public protein profile databases that were used for initial annotation might contain HMMs that represent polyproteins, which span multiple functional domains, we developed and employed a procedure to identify such profiles which were masked from the subsequent annotation process.
All subsequent profile matches passing a predefined cutoff . were used to generate a new custom profile database, in a process similar to the one used for RdRPs . Only clusters with ≥ 10 sequences, sharing the same functional classification, were used to generate HMMs. This profile set was then supplemented by most of the profiles from the above-mentioned RNAVirDB2020 database, as well as several dozen select profiles from the other databases .
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