A study published in MicrobiomeJ suggests that the gamma-delta T cells shape the gut microbiota in ways that improve mucosa tolerance, which, in turn, help to maintain the gamma-delta T cell population. Read the paper here:
Microbiota was depleted by giving mice broad-spectrum antibiotics in their drinking water consisting of ampicillin, metronidazole, and neomycin at 1 mg/mL, and vancomycin at 0.5 mg/mL. To test the effect of microbiota depletion on oral tolerance, antibiotics were given for 3 days prior to and throughout the 5-day OVA feeding period, then antibiotics were replaced by water prior to immunization.
]. One day after the last dose of the miRNA, a group of mice were euthanized for flow cytometric analysis of the SILP, and another group was fed with OVA for oral tolerance experiments.DSMZ 21040, Type Strain were cultured on Brucella agar for 48 h, suspended in Pre-reduced Anaerobically Sterilized saline to an optical density of approximately 0.6.
Deutschland Neuesten Nachrichten, Deutschland Schlagzeilen
Similar News:Sie können auch ähnliche Nachrichten wie diese lesen, die wir aus anderen Nachrichtenquellen gesammelt haben.
New microbiological insights from the Bowland shale highlight heterogeneity of the hydraulically fractured shale microbiome - Environmental MicrobiomeBackground Hydraulically fractured shales offer a window into the deep biosphere, where hydraulic fracturing creates new microbial ecosystems kilometers beneath the surface of the Earth. Studying the microbial communities from flowback fluids that are assumed to inhabit these environments provides insights into their ecophysiology, and in particular their ability to survive in these extreme environments as well as their influence on site operation e.g. via problematic biofouling processes and/or biocorrosion. Over the past decade, research on fractured shale microbiology has focused on wells in North America, with a few additional reported studies conducted in China. To extend the knowledge in this area, we characterized the geochemistry and microbial ecology of two exploratory shale gas wells in the Bowland Shale, UK. We then employed a meta-analysis approach to compare geochemical and 16S rRNA gene sequencing data from our study site with previously published research from geographically distinct formations spanning China, Canada and the USA. Results Our findings revealed that fluids recovered from exploratory wells in the Bowland are characterized by moderate salinity and high microbial diversity. The microbial community was dominated by lineages known to degrade hydrocarbons, including members of Shewanellaceae, Marinobacteraceae, Halomonadaceae and Pseudomonadaceae. Moreover, UK fractured shale communities lacked the usually dominant Halanaerobium lineages. From our meta-analysis, we infer that chloride concentrations play a dominant role in controlling microbial community composition. Spatio-temporal trends were also apparent, with different shale formations giving rise to communities of distinct diversity and composition. Conclusions These findings highlight an unexpected level of compositional heterogeneity across fractured shale formations, which is not only relevant to inform management practices but also provides insight into the ability of diverse microb
Weiterlesen »
Reprogramming Cancer into Antigen Presenting Cells as a Novel ImmunotherapyAbstract. Therapeutic cancer vaccination seeks to elicit activation of tumor-reactive T cells capable of recognizing tumor-associated antigens (TAAs) and eradicating malignant cells. Here, we present a cancer vaccination approach utilizing myeloid lineage reprogramming to directly convert cancer cells into tumor reprogrammed-antigen presenting cells (TR-APCs). Using syngeneic murine leukemia models, we demonstrate that TR-APCs acquire both myeloid phenotype and function, process and present endogenous TAAs, and potently stimulate TAA-specific CD4+ and CD8+ T cells. In vivo TR-APC induction elicits clonal expansion of cancer-specific T cells, establishes cancer-specific immune memory, and ultimately promotes leukemia eradication. We further show that both hematologic cancers and solid tumors, including sarcomas and carcinomas, are amenable to myeloid-lineage reprogramming into TR-APCs. Finally, we demonstrate the clinical applicability of this approach by generating TR-APCs from primary clinical specimens and stimulating autologous patient-derived T cells. Thus, TR-APCs represent a cancer vaccination therapeutic strategy with broad implications for clinical immuno-oncology.
Weiterlesen »
Dead Cells: Return To Castlevania PS4 review - back to the source8/10 I Dead Cells: Return To Castlevania A loving homage to Castlevania and another great slice of DLC for Dead Cells, with the two games working together in perfect harmony 🎮
Weiterlesen »
Researchers optically evoke tachycardia enhanced anxiety-like behaviourIn a recent study published in the journal Nature, researchers devised a non-invasive optogenetic and wearable cardiac pacemaker for accurate and specific control of cardiac rhythm for ≤900 beats per minute (bpm), enabled by micro-light-emitting display (LED) harness and systemic channelrhodopsin delivery.
Weiterlesen »
Frontiers | Myokines derived from contracting skeletal muscle suppress anabolism in MCF7 breast cancer cells by inhibiting mTORThere is strong evidence that physical activity has a profound protective effect against multiple types of cancer. Here, we show that this effect may be mediated by factors released from skeletal muscle during simulated exercise, in situ, which suppress canonical anabolic signaling in breast cancer. We report attenuated growth of MCF7 breast cancer cells in the presence of a rodent-derived exercise conditioned perfusate, independent of prior exercise training. This reduction was concomitant with increased levels of DEPTOR protein and reduced mTOR activity.
Weiterlesen »
Autoantibodies against chemokines post-SARS-CoV-2 infection correlate with disease course - Nature ImmunologyRobbiani and colleagues show that antibodies against specific chemokines are detected in COVID-19 convalescents and may modulate the inflammatory response and disease outcome.
Weiterlesen »