Discovery of an unexpected function of blood immunecells: Their ability to proliferate natimmunol
mice were injected i.p. with 1 mg EdU in 200 µl PBS 4 h before killing, unless otherwise stated. For all experiments involving EdU incorporation, 1 µg of PerCP-Cy5.5-conjugated anti-mouse CD45 was i.v. injected 10 min before killing to distinguish blood circulating and tissue leukocytes . For Csf1r-blocking experiments, 250 µg of anti-mouse Csf1r-blocking antibody or isotype control was injected i.v. 6 and 28 h after DT injection.
For BM cells, femurs and tibias were dissected and cleaned of soft adhering tissue. Distal and proximal ends were opened, and BM cells were flushed out. After centrifugation, cell pellets were resuspended in ice-cold PBS containing 10 mM EDTA and cell suspensions were filtered using a cell strainer to obtain a single-cell suspension.
Lungs, brains, liver and spleen were cut into small pieces with razor blades, and digested for 1 h at 37 °C in HBSS containing 5% vol/vol FBS , 1 mg mlDNase I . After 45 min of digestion, the suspension was flushed using a 18-gauge needle to dissociate aggregates. Ice-cold PBS containing 10 mM EDTA was added to stop the digestion process and cell suspensions were filtered using a cell strainer .
Deutschland Neuesten Nachrichten, Deutschland Schlagzeilen
Similar News:Sie können auch ähnliche Nachrichten wie diese lesen, die wir aus anderen Nachrichtenquellen gesammelt haben.
The X-linked epigenetic regulator UTX controls NK cell-intrinsic sex differences - Nature ImmunologyCheng et al. demonstrate that an extra copy of the X-linked epigenetic regulator UTX in females increases natural killer (NK) cell effector function. As NK cells are critical for antiviral immunity, this may explain decreased severity of viral infections in females compared to males.
Weiterlesen »
Nature agency culls Highland estate deer after landowner failureNatureScot says it killed the deer to protect vulnerable habitats around Loch Choire Estate.
Weiterlesen »
Get our nature fixRachel Massey is the founder of Nature Fix and Other Ways to Walk established to support people’s mental health through nature. A qualified…
Weiterlesen »
Highlands tops nature-watching list as best place to spot wildlife in the UKResearch by Millets hailed the Scottish region’s wealth of wildlife, from wild birds to beasties to abundant heathers.
Weiterlesen »
Hedges planted as Bodenham nature reserve prepares to openPools are also being created at the site, Sarah King, from Herefordshire Wildlife Trust, says.
Weiterlesen »
Nature in danger as mountain forest loss quickensKey mountain habitats are disappearing more quickly due mostly to logging and wildfires.
Weiterlesen »