Next-gen intranasal vaccine takes on measles, mumps, and multiple COVID-19 variants in one shot

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Next-gen intranasal vaccine takes on measles, mumps, and multiple COVID-19 variants in one shot
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itemprop=description content=Researchers developed an intranasal trivalent measles-mumps-SARS-CoV-2 vaccine that provides robust protection against multiple coronavirus variants. Leveraging the established measles-mumps-rubella platform, this vaccine demonstrates broad and adaptable immune responses, especially against Delta and Omicron variants.

By Vijay Kumar MalesuOct 8 2023Reviewed by Susha Cheriyedath, M.Sc. In a recent study published in the journal PNAS, a group of researchers developed an intranasal measles-mumps-SARS-CoV-2 spike protein vaccine that offers broad, durable protection against major Severe Acute Respiratory Syndrome Coronavirus 2 variants, leveraging the proven safety of the measles-mumps-rubella vaccine platform.

About the study The research delved into the comprehensive analysis and development of modified measles and mumps viruses , recombinant Mumps Virus , and rMuV-JL2) that express the SARS-CoV-2 six prolines proteins. Procedures for growth curves, virus preparation, purification, and various assays were implemented.

Recovery and characterization of rMuV-JL1-Delta-preS-6P expressing the six proline-stabilized prefusion spike of SARS-CoV-2 Delta variant. Strategy for insertion of preS-6P of the Delta variant into the P and M gene junction in the MuV-JL1 genome. The plaque morphology of rMuV-JL1 and rMuV-JL1-Delta-preS-6P in Vero CCL81 cells at day 5. rMuV-JL1-Delta-preS-6P exhibits delayed syncytia formation in Vero CCL81 cells . Replication kinetics of recombinant viruses in Vero CCL81 cells at an MOI of 0.

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