Novel multi-epitope subunit vaccine induces robust immune response against Epstein-Barr virus NatureComms epitope subunitvaccine immuneresponse epsteinbarrvirus EBV virology epsteinbarr
By Dr. Sanchari Sinha Dutta, Ph.D.Aug 14 2023Reviewed by Sophia Coveney Scientists from Australia and the USA have developed a lymph node-targeted multi-epitope subunit vaccine that induces strong humoral and cellular immune responses against Epstein-Barr virus in mice. The study is published in the journal Nature Communications.
Vaccine design Scientists analyzed known human CD8+ T cell epitopes from EBV antigens to design a polyepitope vaccine immunogen that incorporates 20 CD8+ T cell epitopes into an engineered protein immunogen. Related StoriesTo further increase vaccine-induced immune response, an Amphiphile-modified CpG DNA adjuvant was incorporated into the vaccine. This adjuvant was designed to increase lymph node delivery and accumulation of vaccine antigen and adjuvant.
The findings revealed that vaccination with Amphiphile-CpG adjuvant induces significantly higher viral antigen-specific cytokine-producing CD8+ T cells compared to vaccination with soluble CpG. The Amphiphile-CpG vaccine-induced T cells exhibited robust polyfunctional effector phenotype. Moreover, vaccination with Amphiphile-CpG induced three times more gp350-specific cytokine-producing CD4+ T cells than soluble CpG.
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