How tomato plants defend themselves against a devastating 'young' Southern African virus has now been investigated at a molecular genetics level for the first time by researchers at the University of Johannesburg (UJ).
that harbor the Ty-1 gene are infected with the relatively unknown tomato curly stunt virus . They found a link between tolerance to ToCSV, a plant defense called viral DNA methylation, and Ty-1 gene activity. The research is published inTo find out more about how that happens, they used lab techniques called bisulfite conversion and PCR amplification, coupled with a next-generation whole genome virus sequencing approach.
"Geminiviruses like ToCSV do not have the capacity to make copies of themselves, and then to spread elsewhere. They are completely reliant on the plant for that. The virus has to trick the plant into 'thinking' that the plant is making more of its own DNA. The susceptible variety has a number of defenses in its toolbox against other pathogens, but it cannot overcome ToCSV, says Allie. ToCSV can fully infect this variety.
"Let's compare a cell of a plant that is susceptible to ToCSV, with a cell of a tomato plant that can tolerate ToCSV to some extent. In the tolerant plant cell the Ty-1 gene is very active.
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