Nature research paper: Selective TnsC recruitment enhances the fidelity of RNA-guided transposition
= 3 independent biological replicates. The absolute sample numbers shown in= 10, 20, 30, 40, 30, 20, and 10 for mismatch position 1, 2, 3, 4–29, 30, 31, and 32, respectively., Representative cryo-micrographs for the TnsC•ATP heptamer at various defoci; the corresponding CTF is shown ., Representative reference-free 2D class averages with four types of views identified: top and bottom views, a lateral side view and intermediate views.
Extended Data Fig. 6 Inter-monomer interactions, biochemical characterization, and TnsC heptamer formation., Purification and heptamer assembly experiments with ATPase mutants of TnsC. SDS–PAGE analysis and SEC chromatograms are shown for the indicated mutants, with TnsC monomer and heptamer peaks indicated. Mutations in the Walker A , Walker B , and arginine finger motifs prevent formation of stable heptamers.
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