Study: Caterpillar traces repel spidermites and may help agriculture SciReports
females, we conducted dual-choice experiments using T-shaped pathways of filter paper . Using disposable micropipettes , 1.75 caterpillar equivalents of acetone extract were applied to an alternately selected branch of each pathway , with control acetone applied to the other branch. We applied each solution dropwise at the junction point to minimize mixing.
After evaporating the solvent from those pathways, we perpendicularly suspended them and introduced an adult female mite at 2 days post-maturation onto the bottom of each pathway using a fine brush and recorded the branch along which the female first walked to the far end. Each female mite and each T-shaped filter paper were used only once, with 19 replicates. Each female mite made a choice within 10 min.
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