A study in the Journal of Neuroinflammation suggests that although COVID-19 is not an active neuroinflammatory disease, it does affect the brain indirectly through the activated inflammatory pathways outside the brain.
The categorical factor sex has two levels: female and male; the reference level is male and factor f2 takes into account PCT level and f3 differences in the WHO grade.
Model 2 was devoted to a more accurate evaluation of the influence of bacterial superinfection on the proteome of COVID patients. For this, we used transformed numerical values of PCT levels f2 = log. As COVID-19 groups had roughly the same proportion of females , sex was introduced into the model as a categorical factor.
For model 2, the significance level alpha was set to guarantee the false discovery rate below ~ 10% for contrast f2. We found that alpha = 0.01 was delivering the required level of Benjamini–Hochberg FDR. The log fold change threshold was set as 0.5.Functional analysis was carried out using R package clusterProfiler. For selecting the most regulated GO terms we applied filter: 2 ≤ term size ≤ 250. Analyses were carried out with Benjamini–Hochberg FDR threshold of 0.3.
For total RNA sequencing, 1 ml CSF was used for RNA purification using miRNeasy Serum/Plasma advanced kit according to the manufacturer`s protocol and the RNA was eluted in 50 μl RNase-free water. The genomic DNA removal was done by using Turbo DNA-free kit according to the manufacturer’s instructions and 50 μl DNA-free RNA was taken out. The RNA was concentrated by adding 50 μl RNase-free water, 10 µl 3 M, pH 5.
Differential expression analysis was performed using DESeq2 in R on the combined gene and circRNA expression levels. Volcano plots, dot plots and PCA plots were done in R. The linear RNA and circRNAs were considered to be significantly differentially expressed with the FDR < 0.05. The enrichment analysis was done using Enrichr
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Research looks to determine prevalence of organ impairment in long-COVID patientsResearch looks to determine prevalence of organ impairment in long-COVID patients Research Coronavirus Disease COVID19 LongCOVID PASC editorjrsm LivUni UniOfHull UniofOxford ucl
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Study on the correlation between B vitamins and breast cancer - Cancer Cell InternationalBackground Relevant studies suggest that serum vitamin level is related to the risk of breast cancer, and dietary pattern and drug supplementation can significantly affect the level of vitamin in the body. Therefore, intervention of vitamin level in the body is expected to be a potential strategy to reduce the risk of breast cancer. However, the current epidemiological findings of serum vitamin levels and breast cancer risk are inconsistent, and the relationship between serum vitamin and breast cancer is still controversial. In this study, we compared the serum vitamin expression levels of healthy people, benign breast patients, and breast cancer patients, and evaluated the relationship between B vitamin levels and breast cancer risk. Methods The study used liquid chromatography-tandem mass spectrometry to determine the serum vitamin levels of 520 people who attended Yunnan Cancer Hospital from September 2020 to December 2020. After screening by exclusion criteria, 38 patients with benign breast diseases, 87 patients with breast cancer and 91 healthy controls were finally included. The kruskal–wallis H test was used to compare the differences in serum vitamin levels of subjects. Χ2 test was used to evaluate the relationship between B vitamin level and age,BMI,TNM staging,Ki-67,Her-2,surgery and chemotherapy, and other baseline characteristics and through binary logistic regression analysis, calculating odds ratio and 95% confidence interval (CI) to evaluate the relationship between B vitamins and breast cancer risk. Conclusion The levels of VitB1 and VitB5 in the serum of breast cancer patients and patients with benign breast diseases were higher than those in the healthy control group, while the expression levels of VitB3 in breast cancer patients were lower than those in the healthy control group and the breast benign disease groups. The level of VitB1 was positively correlated with breast cancer risk. The VitB3 level was negatively correlated with breast cancer r
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No radiotherapy an option for older breast cancer patients, study showsRadiotherapy does not improve survival rates in older patients with early breast cancer, new research suggests.
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Radiotherapy optional for older breast cancer patients, study suggestsRadiotherapy does not improve survival rates in older patients with early breast cancer, a new study has suggested.
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Shropshire transport firm launches pilot scheme to support fallen patients & NHSA new pilot service has been launched in Shropshire aimed at helping to reduce demand on frontline NHS staff, and ensure patients are seen in an even more timely manner.
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Cohort study examines the incidence of SARS-CoV-2 rebound in patients on oral antiviralsResearchers performed a retrospective population-wide cohort study using anonymized data in Hong Kong, China, between February 26 and July 3, 2022.
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