n this interview, Dr. Michelle Chen, Senior Director of Analytical Sciences at Wyatt Technology, talks to NewsMedical about how to use light scattering techniques to analyze proteins for their multi-attribute quantification (MAQ).
Sponsored Content by Waters | Wyatt Technology May 29 2024Reviewed by Olivia Frost insights from industryDr. Michelle ChenSenior Director of Analytical SciencesWyatt Technology In this interview, Dr. Michelle Chen, Senior Director of Analytical Sciences at Wyatt Technology , talks to NewsMedical about how to use light scattering techniques to analyze proteins for their multi-attribute quantification .
Dynamic light scattering measures intensity fluctuations in the scattered light resulting from Brownian motion of the illuminated molecules or particles. By analyzing the time dependence of the fluctuations, one can obtain the translational diffusion coefficient, which is used to calculate the hydrodynamic radius or Rh. Combining DLS with SLS enables estimation of particle concentration, even for heterogeneous sample with multiple size populations.
FFF separates molecules or particles in an open channel, without a stationary phase. During the elution mode, there are two flows: horizontal channel flow and perpendicular cross flow. Smaller particles diffuse against the cross-flow more effectively than larger particles and move further away from the bottom of the channel.
The viral vector analysis module in ASTRA software, provided by Wyatt Technology, calculates several AAV-specific CQAs: the total AAV genomic concentration Vg, the AAV capsid concentration Cp, and Vg/Cp, the full-to-total capsid ratio. ASTRA also calculates the empty AAV concentration, simply Cp minus Vg, and the aggregate content.
What challenges arise in characterizing LNPs compared to proteins and AAVs? Compared to proteins and AAVs, LNPs are relatively less-well characterized at this stage due to their novelty and the limited availability of modality-specific tools for characterization. The complexity of LNPs arises from their large size and heterogeneity.
Automation Capsid Chromatography DNA Dynamic Light Scattering Efficacy ELISA Frequency Gene Gene Therapy Genome Lentivirus Macromolecules Nanoparticle Nanoparticles Polymers Protein QA QC RNA Software Technology Therapeutics Viral Vector
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