A novel blood serum assay to diagnose neurodegenerativediseases NatureMedicine
The IP/RT-QuIC assay efficiently detects minute concentrations of serum α-synuclein seeds, enabling quicker diagnosis of specific neurodegenerative disorders. Credit: Professor Hattori and his team from Juntendo University School of Medicine
In this assay, named immunoprecipitation-based real-time quaking-induced conversion , the α-synuclein seeds are isolated from the patient's serum through immunoprecipitation followed by rapid amplification by real-time quaking-induced conversion . This method is highly sensitive, as it can detect serum α-synuclein seed concentrations as small as 1000pg/ml. This comes as great news since most existing diagnostic methods require cerebrospinal fluid for synuclein detection.
The research team demonstrated that IP/RT-QuIC detected α-synuclein seeds efficiently in patients withand could distinguish them from people without degenerative diseases . Next, they studied the structural properties of the amplified seeds using transmission electron microscopy . Further, when the researchers transduced amplified seeds into the HEK293T cell line stably expressing GFP-fused human α-synuclein with p.A53T mutation and injected seeds into mouse brains , the seeds retained their aggregate forming capacity and diseases-specific seed structure. These aggregates displayed different morphologies depending on the disease type.
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