Mimicking brainplasticity in children to control post-traumatic stress MolPsychiatry
, 32037). All animals were maintained under a light-dark cycle in a temperature and humidity-controlled room. Food and water were available. All procedures described here had been approved by the Comité Institutionnel de Bonnes Pratiques Animales en Recherche of the Research Center of Sainte-Justine Hospital in accordance with the principles published in the Canadian Council on Animal’s Care’s .
The primary antibodies used in this study and their working concentrations are as follows: rabbit monoclonal anti-Hdac2 ; mouse monoclonal anti-PV ; rabbit polyclonal anti-PV ; guinea pig polyclonal anti-PV ; mouse monoclonal anti-gephyrin ; rabbit polyclonal anti-aggrecan ; chicken polyclonal anti-GFP . To label PNNs, a solution of biotin-conjugated lectin Wisteria floribunda was added in the primary antibody solution.
The secondary antibodies used in this study and their working concentrations are as follows: goat anti-chicken Alexa488 conjugated , goat anti-rabbit Alexa633 conjugated , goat anti-mouse Alexa555 conjugated , goat anti-mouse Alexa647 conjugated , goat anti-rabbit Alexa555 conjugated , goat anti-guinea pig Alexa647 conjugated and Alexa 568-conjugated streptavidin .
All immunohistological experiments were performed on at least three different sections per brain region per animal. No mice were excluded from the following analysis.All images were acquired using Leica confocal microscopes . We imaged somatosensory and prefrontal cortex layer 5 and basolateral amygdala using 20X multi-immersion and 63X oil objectives. We focussed on layer 5 of SSCX and PFC because PVcells are more abundant in this layer.
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